[3dem] uneven staining on grids- thanks& problem solved.

[Berith Isaac]

Dear all, 

Thank you for all the replies to my question. I appreciate the time& effort put into trying to help us solve this problem. 

I tried most of the different suggestions and the two things which seemed to make the most difference were:

- Adding a few tens of uliters of 200 proof EtOH to the bell jar during glow discharge (probably helps charging be more effective), as suggested by Dan Shi.

- Drying the grids in the hood (on the edge, so that there's an even flow of air over the grid, which presumably causes the stain to dry in a more even manner), as suggested by Greg Alushin. 

Thanks again!
Berith

Berith Isaac, PhD
Dept. of Molecular and Cellular Biology
Room 305
Harvard University
52 Oxford street
Cambridge, MA 02138, USA
Phone: 617-496-3808
Fax: 617-496-2712

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