[3dem] protein complex crosslinking for EM

[Alexandra Deaconescu]

Dear EM enthusiasts:

I am working on a protein complex that appears to dissociate upon  
dilution for EM grid preparation. I was therefore thinking of using  
some sort of heterobifunctional crosslinker (something other than  
glutaraldehyde that would probably generate a big mess) to stabilize  
the complex and then purify it by gel filtration. I was wondering if  
anyone has and would like to share his/her experiences, or point me  
to some good papers where this has been done before...

I would greatly appreciate your help.

Best,
Alexandra






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