[3dem] how to clean the blotting pad of the vitrobot?

[e.orlova at mail.cryst.bbk.ac.uk]

Dear Guiqing  and Matthias,

I would be very careful if I were you in dealing with infectious agents. I would
 check safety level of the virus you are dealing with and consult with your
biosafety people if you are allowed to handle it in open research space at all.
If virus is airborn then not only you contaminate blotting pads but could
potentially spread it in the Vitrobot chamber and even outside into the room.
You should be concerned with decontamination of the whole Vitrobot chamber
after freezing, most likely using chemicals (e.g. Cavicide). We could discuss
that matter outside of the list, just send me e-mail (mbsherma at utmb.edu).

Good luck!

Michael Sherman

Quoting Matthias Floetenmeyer <m.floetenmeyer at uq.edu.au>:

> Hi Guiqing,
> 
> We just use a layer parafilm in the same shape as the blotting paper  
> between the paper and the sponge pad.
> 
> 
> Regards
> 
> 
> Matthias
> 
> On 24/06/2008, at 04:14, G.-Q. Hu wrote:
> 
> > Dear all,
> >
> > We plan to freeze some pathogenic virus on our vitrobot. But we do  
> > not know how to clean the blotting pad after finishing the freezing.  
> > During the freezing process, the blotting paper get wet and the  
> > black circular sponge pad next to it will absorb the pathogenic  
> > virus.   Could you share your experience about how to clean the  
> > sponge pad in order to kill the virus?
> > Thank you.
> > Guiqing
> > _______________________________________________
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> 
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