[3DEM] Resin sections in a cryo instrument

[David Bhella]

Dear Colleagues,

I am trying to formulate a policy for a new 200 keV FEG cryo TEM we  
are installing, with regards to what should and should not be  
permitted in the column. The instrument is intended primarily for  
cryo and 3D reconstruction by both single particle and tomographic  
methods.
I have canvassed several opinions, particularly with regards to resin  
sections, and received a very polarised response. Many have indicated  
that they do not believe there is any danger of increasing  
contamination, while others are equally adamant that cryo-instruments  
should be used for that purpose only.

Does anyone have any concrete evidence in support of one or other  
viewpoint?
It seems that opinions on this are firmly held, it would be good to  
know if someone has conducted an in-depth investigation.

Many thanks,
D.


Dr David Bhella
MRC Virology Unit
Church Street
Glasgow
G11 5JR

Telephone:  0141-330-3685
Graphics lab:  0141-330-2989
Mobile:  07717-202439

Virus Structure Web Site:
http://www.mrcvu.gla.ac.uk/research/bhellad/virus_structure.htm

Molecular Machines - Images from Virus Research (Art Exhibition):
http://www.molecularmachines.org.uk

Departmental Web Site:
http://www.mrcvu.gla.ac.uk/staff/bhellad.html


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