[3DEM] Outrageous cryo-sectioning idea

[Bill Tivol]

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Dear List,
	In a recent paper from our lab (J. Struct. Biol. 153:241-252), it was 
shown that high-density amorphous ice (HDAI), which is stable at 12 K, 
flows more readily than low-density amorphous ice, the stable form at 
LN2 temperature.  This has me wondering if it would be possible to cut 
better cryosections from HDAI under LHe than can be done with present 
technology.  Of course, I realize that there are great technical 
difficulties in setting up a cryomicrotome at 4 K, and devoting the 
necessary resources to accomplish this is well beyond the capabilities 
of our lab, so I thought I'd put the idea out to the list and see 
whether anyone was interested in pursuing it (once the laughter 
subsides).
Yours,
Bill Tivol, PhD
EM Scientist
Electron Cryo-Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol at caltech.edu
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