[3DEM] Postdoctoral Position

[Edward Egelman]

A postdoctoral position is available in the Egelman laboratory for EM 
studies of helical polymers. We have developed a new methodology of 
single-particle reconstruction of helical polymers (Egelman, 2000), and 
numerous applications have shown that the method can surmount many of 
the problems encountered by disordered, variable and heterogeneous 
filaments (Egelman, 2007). Applications of the method to such polymers 
as Type Three Secretion System needles (Wang et al., 2006), bacterial 
pili (Craig et al., 2006), and myosin thick filaments (Woodhead et al., 
2005) show the enormous power of this approach compared to classical 
helical reconstruction methodologies. Facilities are excellent, with a 
Field Emission Gun TEM and extensive computational resources. The 
candidate might have a background in either cryo-electron microscopy 
itself or image analysis. The University of Virginia is located in 
scenic Charlottesville, Virginia, and Charlottesville has been ranked 
the Best Place to Live in America by Frommer's Cities 
(http://www.usatoday.com/life/lifestyle/2004-03-29-best-cities-main_x.htm). 
Applicants should supply a CV and the name of three references.

 

Craig,L., Volkmann,N., Arvai,A.S., Pique,M.E., Yeager,M., Egelman,E.H., 
and Tainer,J.A. (2006). Type IV Pilus Structure by Cryo-Electron 
Microscopy and Crystallography: Implications for Pilus Functions in 
Pathogenicity. Mol. Cell /in press/.

Egelman,E.H. (2000). A robust algorithm for the reconstruction of 
helical filaments using single-particle methods. Ultramicroscopy /85/, 
225-234.

Egelman,E.H. (2007). The iterative helical real space reconstruction 
method: Surmounting the problems posed by real polymers. J. Struct. 
Biol. /157/, 83-94.

Wang,Y.A., Yu,X., Yip,C., Strynadka,N.C., and Egelman,E.H. (2006). 
Structural Polymorphism in Bacterial EspA Filaments Revealed by Cryo-EM 
and an Improved Approach to Helical Reconstruction. Structure /14/, 
1189-1196.

Woodhead,J.L., Zhao,F.Q., Craig,R., Egelman,E.H., Alamo,L., and 
Padron,R. (2005). Atomic model of a myosin filament in the relaxed 
state. Nature /436/, 1195-1199.

-- 

Edward H. Egelman, Ph.D.

Professor

Dept. of Biochemistry and Molecular Genetics
University of Virginia

phone: 434-924-8210
fax: 434-924-5069
egelman at virginia.edu <mailto:egelman at virginia.edu>
http://www.people.virginia.edu/~ehe2n 
<http://www.people.virginia.edu/%7Eehe2n>

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