[3DEM] imacon flexcolor scanner

[Kasim Sader]

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Hi,

We have done some characterization of our Imacon Flextight 848.  First, 
if you want to scan full micrographs, you will be limited to a greater 
than 9µm step size unless more recent models have larger detectors than 
the 8000 pixel array on the 848.  The other issue is that the gamma 
settings are not correct: scans using a gamma setting of 1 will not 
result in transmittance values.  Imacon/Hasselblad have made a fix for 
this, which we have verified works.  Preliminary MTF measurements using 
Alan Roseman's target (Ultramicroscopy, 96 (2003) 207-218) show better 
MTF values than the Nikon Coolscan 8000ED, though one issue may be that 
the optical setup is changed when changing the resolution.

cheers,

Kasim

Kasim Sader
Institute of Molecular and Cellular Biology
University of Leeds


On 1 Mar 2006, at 15:18, Julie L. Hodgkinson wrote:

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>
> Hi all,
>
> anyone know if the Imacon scanner running with Flexcolor software is 
> good for
> scanning negs of macromolecules for image analysis, high res scanning 
> in other
> words. If so any tips what settings to use would be much appreciated.
>
> cheers,
>
> Jools
> -- 
> Dr. Julie Lynne Hodgkinson, Birkbeck College, University of London, 
> School of
> Crystallography, Room 3076, Malet Street, London, WC1E 7HX. Phone: 020 
> 7631
> 6842
>
>
>
>
>
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