tecnai version 2.1.5

Bob Grassucci bob.grassucci at wadsworth.org
Tue Jun 28 13:32:56 PDT 2005

Hi Angel,
         I am very sorry to hear about your FEG tip.  One thing that 
surprises me about this event is that the user did not realize that Vb was 
open since it is right next to Va.  The sad thing about it is that even if 
he did realize it the software would not allow him to close Vb since it 
would be locked.  I agree that the software should protect the tip at all 
costs and close the column valves when venting the airlock.  I know that if 
I hit the cartridge button V7 and V4 do indeed close.  We have been having 
the opposite problem with venting the airlock that it fails to open the 
vent valve at all unless we hit vent air lock more than one time.  There is 
no substitute for common sense when using these instruments that are still 
on the "bleeding" edge.  Whenever there is a potential for a vacuum 
disaster the number one mantra I instill in the users is protect the tip 
and close the column valves.  If you have to leave the room close the 
valves.  When a tip goes bad there is at least a week of down time.  My 
hope is that you get a good stable tip as a replacement.
         The integration of the TVIPS cameras leaves something to be 
desired but we are slowly working through it.  This is our main 
"bug".  Good luck with your tip replacement and I hope your are up and 
running very soon.

At 01:39 PM 6/28/2005 -0500, Angel M Paredes wrote:
>Hi all,
>Sunday we experienced a rather bad malfunction that people should be aware
>of that destroyed our FEG tip.  On our Polara, the user working with the
>scope decided to remove the MSC to exchange the specimen.  To do this you
>have to vent the airlock.  He pushed the "vent airlock" button.  Since he
>had not closed the column valves, the software I believe was supposed to
>have detected this and closed the column valves for him to protect the
>FEG.  The software unfortunately did not.  What the user did not know was
>that there was an electrical short in valve B that told the software that
>valve B was closed when it was in fact opened.  Instead of telling him to
>close valve B as it should have done, the software detected that valve B
>was already closed and told the user instead to close valve A... which he
>did.  The software then vented the airlock but with valve B opened instead
>of closed, the column was vented and since the software had not closed the
>column valves when the user pushed "vent airlock", the FEG was vented too.
>FEG tip gone.
>We are currently using version 2.1.5 of the user interface.  Has anyone
>out there been experiencing any other bugs with 2.1.5?  Thorsten had
>problems with his system rebooting when a log file was full and I went
>through the same thing during a bugcheck the system decided to perform.
>Another bug we've seen is in low dose.  We use diffraction while in
>search.  Every now and again the search mode goes from looking normal to
>looking really strange and becoming very difficult to align.  When I go
>from diffraction back to regular image mode in search, I find that the
>microscope has gone from a magnification of 52,000x to 52x while in
>diffraction and this is the reason why the search mode goes from looking
>okay to looking strange.  FEI's response is that we are not supposed to be
>using diffraction and experiments in the factory have shown that if one
>switches in and out of diffraction too quickly, it causes the mag to
>change.  All I know is that version 2.1.3 did not have this problem.  Are
>there any other problems I should be made aware of?

Robert Grassucci
Howard Hughes Medical Institute
Wadsworth Center
Empire State Plaza
Albany, NY 12201-0509

bobg at wadsworth.org
Phone: (518)474-5821
Fax:	(518)486-2191

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