[3DEM] Pumping cryo holders

[Henning Stahlberg]

Hello,

The attachment shows our oil-free pumping system "TSH 071" from 
Pfeiffer. In this picture it pumps just one holder, but will be 
extended by adding more T-tubes and valves in a dedicated stand to pump 
several holders. The improvised connection to the tubing is tight. 
Nevertheless, our workshop is currently building a dedicated, clean 
connection. We pump the holders to 1*10-6 mbar with this, measured 
where the gauge is in that picture.

Henning.

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Henning Stahlberg, Ph.D.,
Molecular & Cellular Biology, Briggs Hall 115B,
UC-Davis, 1 Shields Ave., Davis, CA 95616, USA
Tel: +1 (530) 752 82 82      Fax: +1 (530) 752 30 85
mailto:HStahlberg at ucdavis.edu	
http://www.amp.ucdavis.edu	
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On Feb 4, 2004, at 9:44 AM, Christopher Gilpin wrote:

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>
> Hi,
> I was wondering what people were using to pump the dewars of TEM cryo
> holders. I have been using a line to a standard Denton coating unit but
> this is becoming impractical. I am aware of dedicated solutions as
> produced by Gatan but would be interested in how creative users have
> been.
>
> Regards
> Chris
>
> Christopher J. Gilpin Ph.D.
> Assistant Professor
> Director Molecular and Cellular Imaging Facility
> K1.246
> Department of Cell Biology
> University of Texas Southwestern Medical Center
> 5323 Harry Hines Boulevard
> Dallas, Texas 75390-9039
> +1 214 648 2827 Phone
> +1 214 648 6408 Fax
> christopher.gilpin at utsouthwestern.edu
>
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