NS versus cryo
Denis Chretien
denis.chretien at univ-rennes1.fr
Thu Sep 26 02:48:01 PDT 2002
One small comment concerning negative-stain versus cryo-EM of
frozen-hydrated specimens. One of the best advantages of cryo-EM is
that it preserves the aqueous environment of the specimen, not only
water, but also pH or ionic-strength. This is not the case with
negative stain and the overall conformation of the macromolecular
assemblies can be drastically modified by these environmental
changes. If the only goal of three-dimensional reconstruction is to
get "a" structure, then negative-staining is probably fine. However,
if you want to interpret your structure in terms of its function,
then cryo-EM is - to my opinion- the only way to go.
_____________________________________
Denis Chretien
Equipe "Structure et Dynamique du Cytosquelette"
UMR 6026 "Interactions Cellulaires et Moleculaires"
Universite de Rennes 1
Campus de Beaulieu, Bt 13
35042 RENNES Cedex
FRANCE
_____________________________________
tel: +33 (0) 2 23 23 67 64
+33 (0) 6 62 00 84 57
fax: +33 (0) 2 23 23 50 48
email: denis.chretien at univ-rennes1.fr
http://www.sdc.univ-rennes1.fr/
_____________________________________
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