cryo-neg stain

[Sharon G. Wolf]

We've used the procedure according to Adrian et al (Micron Vol.29 pp =
145-160 1998)  with very good results.

Its 16% ammonium molybdate at pH 6.8. The gold-sputtered holey carbon grid =
that is mentioned in the reference above  also helps to keep the particles
from sticking to the carbon and promotes more of them into the holes, but =
of course, some samples do not tolerate the gold, and fall apart. It can =
be worth=20
checking, though.

=97 Sharon=20



>>> Frank Booy <f.booy at ic.ac.uk> 25-Sep-02 3:48:17 PM >>>
While everyone is thinking about cryo-negative stain, what are the =
optimum=20
conditions ie which neg stain and which concentration should be used?

Frank
Frank Booy (Senior Lecturer)
Dept of Biological Sciences,
Room 314a Wolfson Laboratory
Imperial College
Exhibition Road
London SW7 2AY
England
tel 44 (0) 207 5945326
f.booy at ic.ac.uk=20
fax 44 (0) 207 5945317

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