GOURI MAUMZDER, POSTDOCTORAL FELLOWSHIP

Sat Oct 26 11:29:25 PDT 2002

To
Dr.Reiko Oda,

I got my Ph.D degree in crystal structure analysis of
some anti-HIV active nucleoside analogs. Soon after
submission of my Ph.D. thesis I worked on RecX (a RecA
regulatory protein of mol wt 19kDa), its expression,
purification and crystallisation. I went to USA to do
my postdoctoral research work on blood coagulation
protein Prothrombin (72kDa), its crystallisation  and
X-ray diffraction analysis of factor VIIa-TissueFactor
complex using programs X-PLOR and O. At present in
India I am doing collaboration work on crystal
structure analysis of cysteine protease Calotropin
(29kDa) by molecular replacement  using  programs
X-PLOR and O.
I am very much interested in your research work. As I
have got  research experience in protein
crystallography right from expression, purification
and crystallization and crystal structure analysis so
I find that my expertise fits your laboratory. I have
great respect for you and your research work. I think
it would be a great  privilege to get a chance to do
postdoctoral research work in your research
laboratory. I am herewith sending my CV for your kind
consideration. I would be grateful to you if you
kindly offer me a postdoctoral fellowship in your
research laboratory.

With kindest regards,
Yours sincerely, 
Gouri Mazumder.

Name:             Dr.Gouri Mazumder.

Father's name:    Sunil Mazumder.

Date of Birth:    25th september,1969

Present Address:  Materials Science Department,  
                  Indian Association For The  
                  Cultivation of Science,Jadavpur,  
                  Calcutta -700032,India

Educations:

___________________________________________________
Exam        Univ               Year           Class
___________________________________________________
B.Sc.       Gauhati Univ       1994          second
M.Sc.       Gauhati Univ       1996          second
Ph.D.       Jadavpur Univ      2001(September)
___________________________________________________

Experience: Research experience in molecular biology
in studying structure-function relationships of
drugs,proteins and enzymes by X-ray diffraction and
physicochemical methods. I got my Ph.D. degree under
the joint supervision of Prof. M.De of Materials
Science Department, Indian Association for the
Cultivation of Science, Jadavpur, Calcutta-700032,
India and Dr.Anuradha Mukhopadhyay, Physics
Department,Jadavpur University, Jadavpur,
Calcutta-700032,India.    

Research for Ph.D. degree :  

My Ph.D. thesis was on crystal structure analysis of 
some anti-HIV active nucleoside analogs by X-ray
diffraction to study molecular mechanism of drug
action at the protein and gene level. 

Research Specialisation:
Period January 2001-September 2001 

In India after submission of my Ph.D. thesis I worked
on expression, purification and crystallisation of
RecX (19KDa) regulatory protein of RecA under Dr.Amit
Das in the department of Biotechnology of Indian
Institute of Technology, Kharagpur, India.

Postdoctoral research in USA:
Period October 2001- September 2002  

In USA I did my postdoctoral research work on some
blood coagulation proteins Prothrombin (MW 72000) and
FVIIa/TF complex in order to crystallise and determine
structure by X-ray diffraction in the department of
Hematology and Oncology in Saint Louis University. I
screened a wide range of crystallisation conditions
for crystallisation of prothrombin with inhibitor
complex using the sparse-matrix method and  got
crystallising conditions and crystals. I also worked
on 2.8Å resolution data for FVIIa/TF complex  in order
to refine the structure using programs  X-PLOR and
‘O’.

Continuation of research in Protein Crystallography:
Period October 2002- in India

In India at present I am doing collaboration work with
Prof.Ashoke Banerjee,Head of the Department of
Biophysics,Bose Institute,Calcutta on crystal
structure analysis of cysteine protease
calotropin(mol. wt 29kDa) by molecular replacement
using the program X-PLOR and interpreting the electron
density map using the program ‘O’.

Expertise : 

A) Crystallisation and Crystal structure analysis of
small molecules by X-ray  diffraction methods.

B) Crystal structure analysis of protein molecules by
X-ray diffraction methods.

C) Protein expression, purification and
crystallisation by dialysis and vapour
diffusion(hanging/sitting drop method).

D) Basic biochemical characterisation (quality
control) of the protein samples to crystallise.

E) Optimisation of crystallising conditions to produce
crystals suitable for X-ray diffraction.

F) Experience in the use of softwares like X-PLOR,CNS
and O.

Three references and addresses:

1.Prof.Madhusudhan De
Department of Materials Science
Indian Association For the Cultivation of Science
Jadavpur
Calcutta
msmd at mahendra.iacs.res.in

2.Prof.Ashoke Banerjee
Head of the Department of Biophysics
Bose Institute
Calcutta
abbp1 at yahoo.com

3.Dr.Amit Das
Department of Biotechnology
Indian Institute of Technology
Kharagpur
amitk at hijli.iitkgp.ernet.in

              List of Publications
              ____________________

  1.  G.Mazumder, M.De, A.Mukhopadhyay,    
  A.K.Das,S.K.Mazumdar and R.F.Scinazi
  1-(Ethoxymethyl)-6-(Phenyl Selenyl)-5-Ethyl 
  Uracil; a nucleoside analog,
  Journal of Chemical Crystallography (1999)
  29,837-839

  2.  G.Mazumder, M.De, A.Mukhopadhyay, A.K.Das,
  S.K.Mazumdar and G.Bocili
  9-[1-(2-Hydroxy)-3-Hydroxy Propyl Guanine ; a
  nucleoside analog
  Journal of Chemical Crystallography (1999) 29,
  1137-1139

  3.   G.Mazumder, M.De, A.Mukhopadhyay,  
  A.K.Das,S.K.Mazumdar, G.Bocili and Van Aerschot
  9-(r)-[6-(r)-Hydroxymethyl-1-Oxa-4- 
  Thiacyclohexan-2-yl Hypoxanthine-Water(4/3);  a  
  nucleoside analog
  Acta Cryst.(2001) C57, 560-561

  4.   G.Mazumder, M.De, A.Mukhopadhyay,  
  A.K.Das,S.K.Mazumdar and G.Bocili
  Ethyl-3-2'-deoxyuridin-5-yl)-3-Hydroxy-2 
  Iodopropanoate; a nucleoside analog
  Acta Cryst. (2000) C56, 494-495

  5.   G.Mazumder, M.De, A.Mukhopadhyay
  S.K.Mazumdar and A.K.Das
  Oxonium 5-Aminouracil-6-Sulphonate Hydrate
  Acta Cryst. (2001) C57, 215-217

  6.   G.Mazumder, K.Bhttachraya, S.K.Mazumdar  
  et al
  Flemiculosin, a novel chalcone
  Acta Cryst. (1999) C55, 215-217

__________________________________________________
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